Tris(hydroxymethyl)aminomethane

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  • Identifiers

    CAS number
    77-86-1

    Molecular formula
    C4H11NO3

    SMILES
    C(C(CO)(CO)N)O

    Safety labels

    Irritant
    Irritant

  • Odor profile

    Fragrance
    Odorless 51.32%
    Sweet 45.05%
    Fishy 38.49%
    Alcoholic 32.2%
    Oily 19.48%
    Cooling 19.19%
    Ripe 18.13%
    Mint 17.72%
    Bitter 16.94%
    Solvent 16.55%

     

    Flavor
    Odorless 59.27%
    Sweet 45.26%
    Bitter 27.74%
    Sweet-like 27.57%
    Very slight 21.26%
    Mild 20.84%
    Sulfurous 19.74%
    Cauliflower 19.43%
    Soup 19.2%
    Bland 18.96%

     

    Odor impact est.
    Low

  • Properties

    XLogP3-AA
    -2.9

    pKa est.
    5.87 (weak acid)

    Molecular weight
    121.14 g/mol

    Vapor pressure est.

    • hPa @ 20°C
    • hPa @ 25°C

    Evaporation rate
    Ultra slow

    Melting point expt.

    • 171-172 °C

    Boiling point

    • 219-220 °C at 10 mm Hg

    Flash point

    • 181.17 ˚C est.

    Solubility expt.

    • 79.1 mg/mL in ethylene glycol; 26 mg/mL in methanol; 14.6 mg/mL in anhyd ethanol; 22 mg/mL in 95% ethanol; 14 mg/mL in dimethyl formamide; 20 mg/mL in acetone; 0.5 mg/mL in ethyl acetate; 0.4 mg/mL in olive oil; 0.1 mg/mL in cyclohexane; 0.05 mg/mL in chloroform; less than 0.05 mg/mL in carbon tetrachloride
    • In water, 5.50X10+5 mg/L at 25 °C

  • Synonyms

    • Trometamol
    • TROMETHAMINE
    • 77-86-1
    • Tris(Hydroxymethyl)aminomethane
    • Tham
    • Trisamine
    • 2-Amino-2-(hydroxymethyl)-1,3-propanediol
    • Tris buffer
    • Tromethane
    • Tris-base
    • Trisaminol
    • Pehanorm
    • Talatrol
    • Tutofusin tris
    • Tris-steril
    • Addex-tham
    • Trispuffer
    • Trimethylolaminomethane
    • 1,3-Propanediol, 2-amino-2-(hydroxymethyl)-
    • Tris Amino
    • Tris, free base
    • Aminotrimethylolmethane
    • Aminotris(hydroxymethyl)methane
    • Tris(hydroxymethyl)methylamine
    • THAM-E
    • Tris (buffering agent)
    • Tromethamolum
    • Trometamolum
    • 2-Amino-2-methylol-1,3-propanediol
    • NSC-6365
    • DTXSID2023723
    • Methanamine, 1,1,1-tris(hydroxymethyl)-
    • Tris-hydroxymethyl-aminomethan
    • 023C2WHX2V
    • Tris(hydroxymethyly)amino methane
    • NSC6365
    • DTXCID503723
    • NSC65434
    • Tri(hydroxymethyl)aminomethane
    • Tris-Mg(II)-KCl Buffer
    • trometamolo
    • RefChem:6507
    • GlyTouCan:G56296FC
    • B05BB03
    • B05XX02
    • G56296FC
    • 201-064-4
    • Tris
    • 2-Amino-2-(hydroxymethyl)propane-1,3-diol
    • Trizma
    • Tris base
    • Trisamin
    • Tromethanmin
    • Tris(hydroxymethyl)methanamine
    • Trometamole
    • Trizma base
    • bakerbond(tm) cyano (cn)
    • Tris-hydroxymethylaminomethane
    • ABX (ANTIBODY EXCHANGER)
    • Tris-Amino
    • Methylamine, 1,1,1-tris(hydroxymethyl)-
    • NSC 6365
    • 2-(Hydroxymethyl)-2-amino-1,3-propanediol
    • MFCD00004679
    • Trometamol [INN]
    • WP QUAT (STRONG ANION EXCHANGER)
    • 2-Amino-2-hydroxymethyl-1,3-propanediol
    • 126850-03-1
    • 126850-06-4
    • 126850-08-6
    • AMINO (NH2) NARROW-PORE MEDIA-NORMAL PHASE
    • tris(hydroxymethyl) aminomethane
    • 126850-05-3
    • Trometamol(Tris),Proteomics Grade
    • MFCD00132476
    • 1,1,1-tris(hydroxymethyl)methanamine
    • Trometamol(Tris),for molecular biology
    • NCGC00159412-02
    • NCGC00159412-04
    • Tris buffertris-hydroxymethyl-aminomethan
    • Triladyl
    • Trigmo base
    • M02623
    • WLN: Q1XZ1Q1Q
    • 136760-04-8
    • Trometamol(Tris), inverted exclamation markY99.5%
    • 4-Anilino-1-(2-hydroxyethylamino)anthracene-9,11-dione
    • Tromethamine [USAN]
    • (tris(hydroxymethyl)aminomethane)
    • [tris(hydroxymethyl)aminomethane]
    • Caswell No. 036
    • 1, 2-amino-2-(hydroxymethyl)-
    • TRIS(HYDROXY-D-METHYL)AMINO-D2-METHANE, 98 ATOM % D
    • Methanamine,1,1-tris(hydroxymethyl)-
    • Methylamine,1,1-tris(hydroxymethyl)-
    • Trometamolum [INN-Latin]
    • tris(hydroxymethyl)amino methane
    • .beta.-D-ribo-Hexopyranose, 1,6-anhydro-3-deoxy-2-O-(1-methylethyl)-4-O-(phenylmethyl)-
    • CAS-77-86-1
    • Tris(hydroxymethyl)aminomethane, >=99.8%
    • Tris(hydroxymethyl)aminomethane (Tris base)
    • HSDB 3408
    • propane, 2-amino-1,3-dihydroxy-2-hydroxymethyl-
    • Tris(hydroxymethyl)aminomethane (TRIS, Trometamol)
    • EINECS 201-064-4
    • Tris(hydroxymethyl)aminomethane, Electrophoresis Grade
    • Tromethamine [USAN:USP]
    • EPA Pesticide Chemical Code 083901
    • Tris-hydroxymethyl-aminomethan [German]
    • UNII-023C2WHX2V
    • Tromethamin
    • Aminotri(hydroxymethyl)methane
    • tris-buffer
    • tris-amine
    • AI3-03948
    • Tro.meta.mole
    • Trometamol CRS
    • Tris(Hydroxymethyl)-Aminomethane
    • Tro.meta.mol
    • 1gng
    • tris base solution
    • THAM (Standard)
    • Tromethamine (USP)
    • 1h4n
    • Trometamol (JAN/INN)
    • TROMETAMOL [JAN]
    • TRS
    • 77-86-1

  • Applications

    Tris(hydroxymethyl)aminomethane, CAS 77-86-1, is widely used as a buffering agent in biochemistry and molecular biology to maintain near-neutral pH in enzymatic reactions and DNA/RNA workflows; it also serves as a buffer in electrophoresis and other analytical buffers, including common systems such as TBE and TAE; it is used to buffer cell culture media and protein solutions, helping stabilize pH during handling and formulation; in pharmaceutical and cosmetic manufacturing, it can act as a buffering and stabilizing agent in formulations and during production; and in industrial manufacturing, it is employed as a pH adjuster and processing aid in chemical synthesis and analytical applications.

    gpt-5-nano

  • Solubility @25˚C

    Solvent Solubility (g/L)
    ethanol 4.32
    methanol 16.35
    isopropanol 1.29
    water 507.46
    ethyl acetate 0.26
    n-propanol 5.55
    acetone 0.3
    n-butanol 2.47
    acetonitrile 0.3
    DMF 9.48
    toluene 0.04
    isobutanol 1.88
    1,4-dioxane 0.8
    methyl acetate 0.32
    THF 1.49
    2-butanone 0.57
    n-pentanol 0.61
    sec-butanol 2.02
    n-hexane 0.07
    ethylene glycol 33.52
    NMP 24.24
    cyclohexane 0.21
    DMSO 19.66
    n-butyl acetate 0.2
    n-octanol 0.36
    chloroform 0.17
    n-propyl acetate 0.16
    acetic acid 16.58
    dichloromethane 0.16
    cyclohexanone 1.08
    propylene glycol 18.89
    isopropyl acetate 0.19
    DMAc 8.71
    2-ethoxyethanol 4.02
    isopentanol 0.86
    n-heptane 0.25
    ethyl formate 0.55
    1,2-dichloroethane 0.14
    n-hexanol 0.73
    2-methoxyethanol 14.07
    isobutyl acetate 0.09
    tetrachloromethane 0.21
    n-pentyl acetate 0.24
    transcutol 3.89
    n-heptanol 0.82
    ethylbenzene 0.04
    MIBK 0.28
    2-propoxyethanol 2.89
    tert-butanol 0.84
    MTBE 0.21
    2-butoxyethanol 2.59
    propionic acid 6.14
    o-xylene 0.1
    formic acid 134.21
    diethyl ether 0.3
    m-xylene 0.07
    p-xylene 0.05
    chlorobenzene 0.04
    dimethyl carbonate 0.4
    n-octane 0.09
    formamide 63.49
    cyclopentanone 1.83
    2-pentanone 0.42
    anisole 0.08
    cyclopentyl methyl ether 0.78
    gamma-butyrolactone 2.6
    1-methoxy-2-propanol 5.29
    pyridine 0.43
    3-pentanone 0.47
    furfural 2.42
    n-dodecane 0.03
    diethylene glycol 7.5
    diisopropyl ether 0.11
    tert-amyl alcohol 1.32
    acetylacetone 0.4
    n-hexadecane 0.03
    acetophenone 0.19
    methyl propionate 0.62
    isopentyl acetate 0.18
    trichloroethylene 0.21
    n-nonanol 0.35
    cyclohexanol 1.66
    benzyl alcohol 0.29
    2-ethylhexanol 0.42
    isooctanol 0.56
    dipropyl ether 0.38
    1,2-dichlorobenzene 0.06
    ethyl lactate 0.83
    propylene carbonate 0.94
    n-methylformamide 10.55
    2-pentanol 0.91
    n-pentane 0.13
    1-propoxy-2-propanol 1.86
    1-methoxy-2-propyl acetate 0.52
    2-(2-methoxypropoxy) propanol 1.55
    mesitylene 0.08
    ε-caprolactone 1.3
    p-cymene 0.13
    epichlorohydrin 0.74
    1,1,1-trichloroethane 0.08
    2-aminoethanol 33.87
    morpholine-4-carbaldehyde 8.29
    sulfolane 12.46
    2,2,4-trimethylpentane 0.08
    2-methyltetrahydrofuran 0.9
    n-hexyl acetate 0.25
    isooctane 0.04
    2-(2-butoxyethoxy)ethanol 2.14
    sec-butyl acetate 0.11
    tert-butyl acetate 0.25
    decalin 0.05
    glycerin 58.04
    diglyme 2.89
    acrylic acid 9.0
    isopropyl myristate 0.11
    n-butyric acid 3.22
    acetyl acetate 0.21
    di(2-ethylhexyl) phthalate 0.26
    ethyl propionate 0.2
    nitromethane 4.32
    1,2-diethoxyethane 0.26
    benzonitrile 0.12
    trioctyl phosphate 0.27
    1-bromopropane 0.11
    gamma-valerolactone 3.75
    n-decanol 0.24
    triethyl phosphate 0.36
    4-methyl-2-pentanol 0.38
    propionitrile 0.36
    vinylene carbonate 0.74
    1,1,2-trichlorotrifluoroethane 6.17
    DMS 0.26
    cumene 0.05
    2-octanol 0.36
    2-hexanone 0.17
    octyl acetate 0.16
    limonene 0.24
    1,2-dimethoxyethane 1.22
    ethyl orthosilicate 0.36
    tributyl phosphate 0.24
    diacetone alcohol 1.33
    N,N-dimethylaniline 0.17
    acrylonitrile 0.51
    aniline 0.29
    1,3-propanediol 17.71
    bromobenzene 0.03
    dibromomethane 0.13
    1,1,2,2-tetrachloroethane 0.28
    2-methyl-cyclohexyl acetate 0.27
    tetrabutyl urea 0.42
    diisobutyl methanol 0.33
    2-phenylethanol 0.32
    styrene 0.03
    dioctyl adipate 0.19
    dimethyl sulfate 1.55
    ethyl butyrate 0.27
    methyl lactate 2.87
    butyl lactate 0.91
    diethyl carbonate 0.16
    propanediol butyl ether 2.84
    triethyl orthoformate 0.37
    p-tert-butyltoluene 0.14
    methyl 4-tert-butylbenzoate 0.77
    morpholine 2.39
    tert-butylamine 0.43
    n-dodecanol 0.18
    dimethoxymethane 1.39
    ethylene carbonate 0.55
    cyrene 3.91
    2-ethoxyethyl acetate 0.33
    2-ethylhexyl acetate 0.16
    1,2,4-trichlorobenzene 0.1
    4-methylpyridine 0.2
    dibutyl ether 0.16
    2,6-dimethyl-4-heptanol 0.33
    DEF 1.61
    dimethyl isosorbide 1.99
    tetrachloroethylene 0.42
    eugenol 1.01
    triacetin 0.51
    span 80 1.93
    1,4-butanediol 4.32
    1,1-dichloroethane 0.07
    2-methyl-1-pentanol 0.39
    methyl formate 2.71
    2-methyl-1-butanol 1.52
    n-decane 0.06
    butyronitrile 0.3
    3,7-dimethyl-1-octanol 0.27
    1-chlorooctane 0.09
    1-chlorotetradecane 0.04
    n-nonane 0.07
    undecane 0.04
    tert-butylcyclohexane 0.04
    cyclooctane 0.12
    cyclopentanol 3.13
    tetrahydropyran 0.61
    tert-amyl methyl ether 0.35
    2,5,8-trioxanonane 2.36
    1-hexene 0.09
    2-isopropoxyethanol 1.3
    2,2,2-trifluoroethanol 15.0
    methyl butyrate 0.29

    Scent© AI

1 4

IFRA standard 51

Recommendation
No restriction
Maximum acceptable concentrations in the finished product (%)
Category 1
Products applied to the lips
 No restriction Category 7A
Rinse-off products applied to the hair with some hand contact
 No restriction
Category 2
Products applied to the axillae
 No restriction Category 7B
Leave-on products applied to the hair with some hand contact
 No restriction
Category 3
Products applied to the face/body using fingertips
 No restriction Category 8
Products with significant anogenital exposure
 No restriction
Category 4
Products related to fine fragrance
 No restriction Category 9
Products with body and hand exposure, primarily rinse off
 No restriction
Category 5A
Body lotion products applied to the body using the hands (palms), primarily leave on
 No restriction Category 10A
Household care products with mostly hand contact
 No restriction
Category 5B
Face moisturizer products applied to the face using the hands (palms), primarily leave on
 No restriction Category 10B
Household care products with mostly hand contact, including aerosol/spray products (with potential leave-on skin contact)
 No restriction
Category 5C
Hand cream products applied to the hands using the hands (palms), primarily leave on
 No restriction Category 11A
Products with intended skin contact but minimal transfer of fragrance to skin from inert substrate without UV exposure
 No restriction
Category 5D
Baby Creams, baby Oils and baby talc
 No restriction Category 11B
Products with intended skin contact but minimal transfer of fragrance to skin from inert substrate with potential UV exposure
 No restriction
Category 6
Products with oral and lip exposure
 No restriction Category 12
Products not intended for direct skin contact, minimal or insignificant transfer to skin
 No restriction