Ninhydrin

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  • Identifiers

    CAS number
    485-47-2

    Molecular formula
    C9H6O4

    SMILES
    C1=CC=C2C(=C1)C(=O)C(C2=O)(O)O

  • Odor profile

    Fragrance
    Sweet 50.54%
    Odorless 40.97%
    Caramellic 37.98%
    Burnt 33.18%
    Phenolic 32.59%
    Spicy 29.57%
    Bitter 25.52%
    Balsamic 22.89%
    Creamy 22.65%
    Vanilla 22.62%

     

    Flavor
    Bitter 69.39%
    Odorless 26.96%
    Balsam 22.71%
    Sweet 21.91%
    Fenugreek 21.18%
    Very mild 20.85%
    Sweet-like 20.8%
    Urine 20.0%
    Extremely sweet 19.91%
    Cotton candy 19.83%

     

    Odor impact est.
    Medium

  • Properties

    XLogP3-AA
    0.1

    pKa est.
    7.01 (neutral)

    Molecular weight
    178.14 g/mol

    Vapor pressure est.

    • hPa @ 20°C
    • hPa @ 25°C

    Evaporation rate
    Ultra slow

    Boiling point est.
    348°C

    Melting point expt.

    • 466 to 469 °F (Decomposes) (NTP, 1992)

    Flash point

    • 177.16 ˚C est.

    Solubility expt.

    • 1 to 5 mg/mL at 68 °F (NTP, 1992)
    • Solubility in water: soluble

  • Synonyms

    • NINHYDRIN
    • 485-47-2
    • Ninhydrin hydrate
    • 1,2,3-Indantrione monohydrate
    • 1H-Indene-1,3(2H)-dione, 2,2-dihydroxy-
    • Ninhydrine
    • 2,2-Dihydroxy-1H-indene-1,3(2H)-dione
    • 2,2-Dihydroxy-1,3-indandione
    • 2,2-Dihydroxyindane-1,3-dione
    • Triketohydrindene hydrate
    • 2,2-dihydroxyindene-1,3-dione
    • 2,2-Dihydroxy-1,3-indanedione
    • 1,2,3-Indantrione, 2-hydrate
    • CCRIS 4849
    • Ninhydrin Spray
    • 1,3-Indandione, 2,2-dihydroxy-
    • Trioxohydrindene monohydrate
    • EINECS 207-618-1
    • HCL6S9K23A
    • MFCD00003791
    • BRN 1910963
    • 2,2-dihydroxy-2,3-dihydro-1H-indene-1,3-dione
    • DTXSID7025716
    • CHEBI:86374
    • AI3-04464
    • triketohydrinden hydrate
    • NINHYDRIN [MI]
    • DTXCID605716
    • CHEMBL1221925
    • 4-07-00-02786 (Beilstein Handbook Reference)
    • 1,2,3-Triketohydrindene Monohydrate
    • 1H-Indene-1,2,3-trione, 2-hydrate
    • Indantrione Monohydrate
    • Monohydrate, Indantrione
    • UNII-HCL6S9K23A
    • Ningidrin
    • nin-hydrin
    • Indan1,2,3trione
    • Ninhydrin ACS grade
    • Ninhydrin, ACS reagent
    • NINHYDRIN [INCI]
    • 2,2Dihydroxy1,3indandione
    • Ninhydrin, AR, >=99%
    • SCHEMBL17986
    • MLS002454421
    • 1,2,3Indantrione monohydrate
    • 1,2,3Indantrione, 2hydrate
    • 1,3Indandione, 2,2dihydroxy
    • 3-Indandione, 2,2-dihydroxy-
    • 1,2,3-Triketohydrindene hydrate
    • Ninhydrin, >=95.0% (UV)
    • ALBB-033740
    • HY-D0908
    • 2,2Dihydroxy1Hindene1,3(2H)dione
    • Tox21_200705
    • BBL012206
    • BDBM50324901
    • Ninhydrin, puriss. p.a., >=99%
    • STL163548
    • AKOS004906785
    • 1HIndene1,3(2H)dione, 2,2dihydroxy
    • CS-W018489
    • FN06988
    • 1,2,3-INDANTRIONE, 2 HYDRATE
    • INDANE-1,2,3-TRIONE MONOHYDRATE
    • NCGC00091114-01
    • NCGC00091114-02
    • NCGC00091114-03
    • NCGC00258259-01
    • AC-11764
    • AC-37041
    • AS-14832
    • BP-11883
    • CAS-485-47-2
    • Ninhydrin, p.a., ACS reagent, 99.0%
    • PD119090
    • SMR001372015
    • Ninhydrin spray reagent, 0.2% in ethanol
    • Ninhydrin, SAJ special grade, >=98.0%
    • DB-051560
    • Ninhydrin, ACS reagent, >=98.0% (UV)
    • I0015
    • N0094
    • N0719
    • Ninhydrin, suitable for amino acid detection
    • NS00022220
    • EN300-19191
    • H12009
    • AQ-911/40896973
    • Q421098
    • F0001-2517
    • Ninhydrin, >=98.0%, suitable for amino acid analysis
    • Z104473086
    • 2,3-DIHYDRO-2,2-DIHYDROXY-1,3-DIOXO-1H-INDENE
    • 1,2,3-Indantrione monohydrate; 2,2-Dihydroxy-1,3-indanedione
    • 207-618-1

  • Applications

    Ninhydrin (CAS 485-47-2) is a colorimetric reagent widely used to detect amino acids, notably in analytical chemistry for visualization on thin-layer chromatography and paper chromatography and for amino acid assays in protein hydrolysates. In forensic science, it is the standard reagent for developing latent fingerprints on porous surfaces, producing Ruhemann's purple when amino acids are present. It also supports quality control and process monitoring in the food and fermentation industries by assessing free amino acids and overall amino acid profiles. Additionally, it is employed in biochemical research for qualitative and quantitative amino acid analysis in protein and peptide studies.

    gpt-5-nano

  • Solubility @25˚C

    Solvent Solubility (g/L)
    ethanol 44.69
    methanol 72.22
    isopropanol 29.09
    water 4.45
    ethyl acetate 23.45
    n-propanol 27.76
    acetone 68.6
    n-butanol 26.1
    acetonitrile 23.71
    DMF 388.64
    toluene 6.22
    isobutanol 15.89
    1,4-dioxane 93.62
    methyl acetate 28.56
    THF 143.96
    2-butanone 37.21
    n-pentanol 22.91
    sec-butanol 22.91
    n-hexane 2.38
    ethylene glycol 74.84
    NMP 134.33
    cyclohexane 1.55
    DMSO 378.91
    n-butyl acetate 18.99
    n-octanol 37.04
    chloroform 17.84
    n-propyl acetate 12.96
    acetic acid 18.83
    dichloromethane 20.06
    cyclohexanone 39.02
    propylene glycol 29.37
    isopropyl acetate 19.89
    DMAc 196.57
    2-ethoxyethanol 89.52
    isopentanol 21.02
    n-heptane 3.77
    ethyl formate 21.4
    1,2-dichloroethane 30.22
    n-hexanol 32.77
    2-methoxyethanol 173.72
    isobutyl acetate 6.49
    tetrachloromethane 6.2
    n-pentyl acetate 25.47
    transcutol 108.05
    n-heptanol 32.06
    ethylbenzene 7.5
    MIBK 29.18
    2-propoxyethanol 97.09
    tert-butanol 32.91
    MTBE 22.0
    2-butoxyethanol 63.66
    propionic acid 15.09
    o-xylene 8.73
    formic acid 43.62
    diethyl ether 33.9
    m-xylene 9.2
    p-xylene 7.61
    chlorobenzene 8.64
    dimethyl carbonate 39.39
    n-octane 3.56
    formamide 85.53
    cyclopentanone 75.22
    2-pentanone 39.92
    anisole 19.68
    cyclopentyl methyl ether 38.53
    gamma-butyrolactone 71.84
    1-methoxy-2-propanol 110.49
    pyridine 26.93
    3-pentanone 30.66
    furfural 60.36
    n-dodecane 3.68
    diethylene glycol 136.32
    diisopropyl ether 11.35
    tert-amyl alcohol 26.2
    acetylacetone 56.41
    n-hexadecane 4.16
    acetophenone 22.97
    methyl propionate 36.14
    isopentyl acetate 16.16
    trichloroethylene 33.84
    n-nonanol 33.56
    cyclohexanol 19.81
    benzyl alcohol 21.01
    2-ethylhexanol 18.14
    isooctanol 23.52
    dipropyl ether 21.36
    1,2-dichlorobenzene 11.83
    ethyl lactate 21.89
    propylene carbonate 26.04
    n-methylformamide 105.95
    2-pentanol 22.23
    n-pentane 2.41
    1-propoxy-2-propanol 52.23
    1-methoxy-2-propyl acetate 35.34
    2-(2-methoxypropoxy) propanol 44.89
    mesitylene 8.34
    ε-caprolactone 31.7
    p-cymene 9.48
    epichlorohydrin 84.96
    1,1,1-trichloroethane 13.01
    2-aminoethanol 55.52
    morpholine-4-carbaldehyde 139.3
    sulfolane 134.9
    2,2,4-trimethylpentane 1.84
    2-methyltetrahydrofuran 44.57
    n-hexyl acetate 33.95
    isooctane 1.87
    2-(2-butoxyethoxy)ethanol 67.84
    sec-butyl acetate 9.63
    tert-butyl acetate 24.67
    decalin 2.22
    glycerin 86.41
    diglyme 118.09
    acrylic acid 18.08
    isopropyl myristate 18.22
    n-butyric acid 17.79
    acetyl acetate 14.48
    di(2-ethylhexyl) phthalate 15.28
    ethyl propionate 17.42
    nitromethane 61.61
    1,2-diethoxyethane 34.78
    benzonitrile 16.89
    trioctyl phosphate 17.39
    1-bromopropane 12.45
    gamma-valerolactone 91.8
    n-decanol 26.27
    triethyl phosphate 13.17
    4-methyl-2-pentanol 16.17
    propionitrile 19.0
    vinylene carbonate 21.88
    1,1,2-trichlorotrifluoroethane 68.87
    DMS 25.89
    cumene 7.29
    2-octanol 26.34
    2-hexanone 22.75
    octyl acetate 24.34
    limonene 11.96
    1,2-dimethoxyethane 106.72
    ethyl orthosilicate 12.81
    tributyl phosphate 13.84
    diacetone alcohol 49.09
    N,N-dimethylaniline 20.78
    acrylonitrile 30.97
    aniline 13.99
    1,3-propanediol 61.44
    bromobenzene 6.6
    dibromomethane 13.45
    1,1,2,2-tetrachloroethane 36.82
    2-methyl-cyclohexyl acetate 15.06
    tetrabutyl urea 25.64
    diisobutyl methanol 14.66
    2-phenylethanol 28.5
    styrene 6.7
    dioctyl adipate 19.24
    dimethyl sulfate 76.92
    ethyl butyrate 15.24
    methyl lactate 49.09
    butyl lactate 24.6
    diethyl carbonate 10.36
    propanediol butyl ether 50.62
    triethyl orthoformate 14.42
    p-tert-butyltoluene 10.52
    methyl 4-tert-butylbenzoate 36.93
    morpholine 80.42
    tert-butylamine 17.81
    n-dodecanol 19.53
    dimethoxymethane 179.85
    ethylene carbonate 18.9
    cyrene 45.74
    2-ethoxyethyl acetate 32.04
    2-ethylhexyl acetate 12.95
    1,2,4-trichlorobenzene 13.3
    4-methylpyridine 23.55
    dibutyl ether 20.77
    2,6-dimethyl-4-heptanol 14.66
    DEF 64.73
    dimethyl isosorbide 63.54
    tetrachloroethylene 30.26
    eugenol 43.04
    triacetin 27.52
    span 80 34.5
    1,4-butanediol 34.31
    1,1-dichloroethane 14.84
    2-methyl-1-pentanol 17.44
    methyl formate 75.51
    2-methyl-1-butanol 17.59
    n-decane 5.54
    butyronitrile 18.15
    3,7-dimethyl-1-octanol 21.42
    1-chlorooctane 18.08
    1-chlorotetradecane 8.76
    n-nonane 4.81
    undecane 4.52
    tert-butylcyclohexane 2.24
    cyclooctane 1.48
    cyclopentanol 27.1
    tetrahydropyran 32.78
    tert-amyl methyl ether 22.56
    2,5,8-trioxanonane 83.13
    1-hexene 6.91
    2-isopropoxyethanol 46.81
    2,2,2-trifluoroethanol 21.07
    methyl butyrate 19.6

    Scent© AI

1 4

IFRA standard 51

Recommendation
No restriction
Maximum acceptable concentrations in the finished product (%)
Category 1
Products applied to the lips
 No restriction Category 7A
Rinse-off products applied to the hair with some hand contact
 No restriction
Category 2
Products applied to the axillae
 No restriction Category 7B
Leave-on products applied to the hair with some hand contact
 No restriction
Category 3
Products applied to the face/body using fingertips
 No restriction Category 8
Products with significant anogenital exposure
 No restriction
Category 4
Products related to fine fragrance
 No restriction Category 9
Products with body and hand exposure, primarily rinse off
 No restriction
Category 5A
Body lotion products applied to the body using the hands (palms), primarily leave on
 No restriction Category 10A
Household care products with mostly hand contact
 No restriction
Category 5B
Face moisturizer products applied to the face using the hands (palms), primarily leave on
 No restriction Category 10B
Household care products with mostly hand contact, including aerosol/spray products (with potential leave-on skin contact)
 No restriction
Category 5C
Hand cream products applied to the hands using the hands (palms), primarily leave on
 No restriction Category 11A
Products with intended skin contact but minimal transfer of fragrance to skin from inert substrate without UV exposure
 No restriction
Category 5D
Baby Creams, baby Oils and baby talc
 No restriction Category 11B
Products with intended skin contact but minimal transfer of fragrance to skin from inert substrate with potential UV exposure
 No restriction
Category 6
Products with oral and lip exposure
 No restriction Category 12
Products not intended for direct skin contact, minimal or insignificant transfer to skin
 No restriction