Leupeptin acid

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  • Identifiers

    CAS number
    24125-28-8

    Molecular formula
    C20H38N6O5

    SMILES
    CC(C)C[C@@H](C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCCN=C(N)N)C(=O)O)NC(=O)C

    Safety labels

  • Odor profile

    Fragrance
    Odorless 62.16%
    Cooling 44.11%
    Savory 40.29%
    Meaty 26.12%
    Mint 25.77%
    Fishy 23.75%
    Milky 17.89%
    Cooked 17.15%
    Animal 15.93%
    Alcoholic 15.84%

     

    Flavor
    Bitter 49.66%
    Odorless 47.17%
    Bland 32.65%
    Mild 25.78%
    Sweet-like 20.45%
    Nitrile 19.42%
    Cedarleaf 18.89%
    Oriental 18.56%
    Taco 18.12%
    Yeast 18.12%

     

    Odor impact est.
    Low

  • Properties

    XLogP3-AA
    0.5

    pKa est.
    7.48 (neutral)

    Molecular weight
    442.6 g/mol

    Vapor pressure est.

    • hPa @ 20°C
    • hPa @ 25°C

    Evaporation rate
    Ultra slow

    Boiling point est.
    622°C

    Flash point

    • 254.69 ˚C est.

  • Synonyms

    • Leupeptin acid
    • 24125-28-8
    • Acetyl-L-leucyl-L-leucyl-L-arginine
    • DTXSID70178846
    • L-Arginine, N2-(N-(N-acetyl-L-leucyl)-L-leucyl)-
    • RefChem:924693
    • DTXCID30101337
    • Acetyl-L-leu-L-leu-L-arg
    • (2S)-2-[[(2S)-2-[[(2S)-2-acetamido-4-methylpentanoyl]amino]-4-methylpentanoyl]amino]-5-(diaminomethylideneamino)pentanoic acid
    • Ac-LLR-CHO
    • acetyl-leucyl-leucyl-arginine
    • CHEMBL129157
    • orb1696521
    • SCHEMBL3799123
    • BDBM50286441
    • AKOS030606597
    • PD119630
    • N-Acetyl-L-leucyl-L-leucyl-N~5~-(diaminomethylidene)-L-ornithine
    • (2S)-2-[[(2S)-2-[[(2S)-2-acetamido-4-methyl-pentanoyl]amino]-4-methyl-pentanoyl]amino]-5-guanidino-valeric acid
    • (S)-2-((S)-2-Acetylamino-4-(S)-methyl-pentanoylamino)-4-methyl-pentanoic acid (1-formyl-4-guanidino-butyl)-amide
    • (S)-2-[(S)-2-((S)-2-Acetylamino-4-methyl-pentanoylamino)-4-methyl-pentanoylamino]-5-guanidino-pentanoic acid
    • (S)-2-Acetylamino-4-methyl-pentanoic acid [(R)-1-((S)-1-formyl-4-guanidino-butylcarbamoyl)-3-methyl-butyl]-amide
    • (S)-2-Acetylamino-4-methyl-pentanoic acid [(S)-1-((S)-1-formyl-4-guanidino-butylcarbamoyl)-3-methyl-butyl]-amide
    • (S)-2-acetylamino-4-methyl-pentanoic acid [(S)-2-(1-formyl-4-guanidino-butylamino)-4-methyl-pentanoyl]-amide
    • 2-Acetylamino-4-methyl-pentanoic acid [1-(1-formyl-4-guanidino-butylcarbamoyl)-3-methyl-butyl]-amide
    • 24125-28-8

  • Applications

    Leupeptin acid (CAS 24125-28-8) is a natural product-derived protease inhibitor used primarily as a biochemical research tool to inhibit serine proteases such as trypsin and plasmin, enabling control of proteolysis in experimental workflows. It is commonly employed to prevent proteolytic degradation during protein purification and sample handling, to study protease activity in enzymology assays, and to preserve protein integrity in cell culture; it is also evaluated for use in proteomics sample preparation and may be used as a reagent in enzyme production/formulation to manage protease activity. Use is subject to local regulations and formulation limits.

    gpt-5-nano

  • Solubility @25˚C

    Solvent Solubility (g/L)
    ethanol 5.04
    methanol 11.89
    isopropanol 1.55
    water 8.96
    ethyl acetate 0.4
    n-propanol 2.56
    acetone 1.19
    n-butanol 1.27
    acetonitrile 0.54
    DMF 9.86
    toluene 0.28
    isobutanol 1.22
    1,4-dioxane 1.28
    methyl acetate 0.64
    THF 3.79
    2-butanone 1.06
    n-pentanol 1.55
    sec-butanol 1.17
    n-hexane 0.17
    ethylene glycol 7.95
    NMP 29.19
    cyclohexane 0.09
    DMSO 26.69
    n-butyl acetate 0.5
    n-octanol 0.26
    chloroform 1.04
    n-propyl acetate 0.63
    acetic acid 19.64
    dichloromethane 1.11
    cyclohexanone 2.59
    propylene glycol 9.1
    isopropyl acetate 0.27
    DMAc 17.42
    2-ethoxyethanol 8.47
    isopentanol 0.72
    n-heptane 0.07
    ethyl formate 1.6
    1,2-dichloroethane 0.84
    n-hexanol 0.78
    2-methoxyethanol 13.83
    isobutyl acetate 0.35
    tetrachloromethane 0.36
    n-pentyl acetate 0.34
    transcutol 7.34
    n-heptanol 0.53
    ethylbenzene 0.28
    MIBK 0.33
    2-propoxyethanol 5.04
    tert-butanol 0.93
    MTBE 0.24
    2-butoxyethanol 3.35
    propionic acid 3.8
    o-xylene 0.27
    formic acid 55.69
    diethyl ether 0.29
    m-xylene 0.18
    p-xylene 0.41
    chlorobenzene 0.26
    dimethyl carbonate 0.81
    n-octane 0.04
    formamide 29.34
    cyclopentanone 4.27
    2-pentanone 0.6
    anisole 0.45
    cyclopentyl methyl ether 0.94
    gamma-butyrolactone 6.44
    1-methoxy-2-propanol 5.14
    pyridine 1.98
    3-pentanone 0.62
    furfural 8.09
    n-dodecane 0.02
    diethylene glycol 9.03
    diisopropyl ether 0.08
    tert-amyl alcohol 0.95
    acetylacetone 0.86
    n-hexadecane 0.02
    acetophenone 1.01
    methyl propionate 0.81
    isopentyl acetate 0.45
    trichloroethylene 1.45
    n-nonanol 0.29
    cyclohexanol 1.01
    benzyl alcohol 1.59
    2-ethylhexanol 0.35
    isooctanol 0.33
    dipropyl ether 0.36
    1,2-dichlorobenzene 0.3
    ethyl lactate 1.52
    propylene carbonate 2.74
    n-methylformamide 8.33
    2-pentanol 0.49
    n-pentane 0.09
    1-propoxy-2-propanol 2.28
    1-methoxy-2-propyl acetate 0.95
    2-(2-methoxypropoxy) propanol 2.61
    mesitylene 0.11
    ε-caprolactone 3.11
    p-cymene 0.13
    epichlorohydrin 3.7
    1,1,1-trichloroethane 0.38
    2-aminoethanol 10.66
    morpholine-4-carbaldehyde 13.55
    sulfolane 18.83
    2,2,4-trimethylpentane 0.03
    2-methyltetrahydrofuran 1.57
    n-hexyl acetate 0.35
    isooctane 0.01
    2-(2-butoxyethoxy)ethanol 3.88
    sec-butyl acetate 0.34
    tert-butyl acetate 0.27
    decalin 0.06
    glycerin 22.55
    diglyme 5.37
    acrylic acid 6.07
    isopropyl myristate 0.11
    n-butyric acid 2.56
    acetyl acetate 0.37
    di(2-ethylhexyl) phthalate 0.43
    ethyl propionate 0.55
    nitromethane 7.64
    1,2-diethoxyethane 0.65
    benzonitrile 0.57
    trioctyl phosphate 0.29
    1-bromopropane 0.39
    gamma-valerolactone 12.19
    n-decanol 0.21
    triethyl phosphate 0.53
    4-methyl-2-pentanol 0.24
    propionitrile 0.47
    vinylene carbonate 2.42
    1,1,2-trichlorotrifluoroethane 12.68
    DMS 0.58
    cumene 0.21
    2-octanol 0.22
    2-hexanone 0.9
    octyl acetate 0.2
    limonene 0.15
    1,2-dimethoxyethane 4.65
    ethyl orthosilicate 0.51
    tributyl phosphate 0.4
    diacetone alcohol 1.39
    N,N-dimethylaniline 0.63
    acrylonitrile 0.92
    aniline 0.8
    1,3-propanediol 8.04
    bromobenzene 0.19
    dibromomethane 0.71
    1,1,2,2-tetrachloroethane 1.65
    2-methyl-cyclohexyl acetate 0.59
    tetrabutyl urea 0.73
    diisobutyl methanol 0.13
    2-phenylethanol 0.91
    styrene 0.26
    dioctyl adipate 0.29
    dimethyl sulfate 3.03
    ethyl butyrate 0.47
    methyl lactate 2.73
    butyl lactate 1.18
    diethyl carbonate 0.34
    propanediol butyl ether 3.88
    triethyl orthoformate 0.56
    p-tert-butyltoluene 0.11
    methyl 4-tert-butylbenzoate 0.97
    morpholine 3.12
    tert-butylamine 0.35
    n-dodecanol 0.15
    dimethoxymethane 2.33
    ethylene carbonate 1.68
    cyrene 7.05
    2-ethoxyethyl acetate 0.67
    2-ethylhexyl acetate 0.36
    1,2,4-trichlorobenzene 0.47
    4-methylpyridine 1.22
    dibutyl ether 0.19
    2,6-dimethyl-4-heptanol 0.13
    DEF 1.7
    dimethyl isosorbide 4.04
    tetrachloroethylene 1.47
    eugenol 1.61
    triacetin 0.76
    span 80 1.9
    1,4-butanediol 4.75
    1,1-dichloroethane 0.41
    2-methyl-1-pentanol 1.01
    methyl formate 5.47
    2-methyl-1-butanol 0.9
    n-decane 0.03
    butyronitrile 0.47
    3,7-dimethyl-1-octanol 0.18
    1-chlorooctane 0.09
    1-chlorotetradecane 0.04
    n-nonane 0.03
    undecane 0.02
    tert-butylcyclohexane 0.05
    cyclooctane 0.04
    cyclopentanol 2.27
    tetrahydropyran 0.83
    tert-amyl methyl ether 0.3
    2,5,8-trioxanonane 3.68
    1-hexene 0.39
    2-isopropoxyethanol 3.33
    2,2,2-trifluoroethanol 8.74
    methyl butyrate 1.0

    Scent© AI

1 4

IFRA standard 51

Recommendation
No restriction
Maximum acceptable concentrations in the finished product (%)
Category 1
Products applied to the lips
 No restriction Category 7A
Rinse-off products applied to the hair with some hand contact
 No restriction
Category 2
Products applied to the axillae
 No restriction Category 7B
Leave-on products applied to the hair with some hand contact
 No restriction
Category 3
Products applied to the face/body using fingertips
 No restriction Category 8
Products with significant anogenital exposure
 No restriction
Category 4
Products related to fine fragrance
 No restriction Category 9
Products with body and hand exposure, primarily rinse off
 No restriction
Category 5A
Body lotion products applied to the body using the hands (palms), primarily leave on
 No restriction Category 10A
Household care products with mostly hand contact
 No restriction
Category 5B
Face moisturizer products applied to the face using the hands (palms), primarily leave on
 No restriction Category 10B
Household care products with mostly hand contact, including aerosol/spray products (with potential leave-on skin contact)
 No restriction
Category 5C
Hand cream products applied to the hands using the hands (palms), primarily leave on
 No restriction Category 11A
Products with intended skin contact but minimal transfer of fragrance to skin from inert substrate without UV exposure
 No restriction
Category 5D
Baby Creams, baby Oils and baby talc
 No restriction Category 11B
Products with intended skin contact but minimal transfer of fragrance to skin from inert substrate with potential UV exposure
 No restriction
Category 6
Products with oral and lip exposure
 No restriction Category 12
Products not intended for direct skin contact, minimal or insignificant transfer to skin
 No restriction