4-(2-Hydroxyethyl)-1-piperazineethanesulfonic acid

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  • Identifiers

    CAS number
    7365-45-9

    Molecular formula
    C8H18N2O4S

    SMILES
    C1CN(CCN1CCO)CCS(=O)(=O)O

    Safety labels

  • Odor profile

    Fragrance
    Odorless 84.85%
    Savory 24.08%
    Fishy 22.73%
    Meaty 16.36%
    Cooked 16.04%
    Popcorn 14.44%
    Cooling 14.09%
    Alcoholic 12.59%
    Roasted 12.0%
    Animal 11.11%

     

    Flavor
    Bitter 84.17%
    Odorless 36.1%
    Bland 30.0%
    Sweet-like 21.87%
    Nitrile 20.12%
    Cedarleaf 19.75%
    Alkaline 19.49%
    Mild 19.4%
    Bouillon 19.12%
    Taco 19.03%

     

    Odor impact est.
    Low

  • Properties

    XLogP3-AA
    -4.0

    pKa est.
    7.54 (neutral)

    Molecular weight
    238.31 g/mol

    Vapor pressure est.

    • hPa @ 20°C
    • hPa @ 25°C

    Evaporation rate
    Ultra slow

    Boiling point est.
    570°C

    Flash point

    • 220.62 ˚C est.

  • Synonyms

    • HEPES
    • 7365-45-9
    • 4-(2-Hydroxyethyl)-1-piperazineethanesulfonic acid
    • 1-Piperazineethanesulfonic acid, 4-(2-hydroxyethyl)-
    • 4-(2-Hydroxyethyl)-1-piperazineethane sulfonic acid
    • hydroxyethylpiperazine ethane sulfonic acid
    • RWW266YE9I
    • CHEBI:42334
    • NSC-166663
    • NSC166663
    • Monosodium Salt, HEPES
    • Salt, HEPES Monosodium
    • HEPES solution
    • N 2 Hydroxyethylpiperazine N' 2' ethanesulfonic Acid
    • N-2-Hydroxyethylpiperazine-N'-2'-ethanesulfonic Acid
    • RefChem:783545
    • CHEBI:46756
    • Hydroxyethyl Piperazine Ethanesulfonic Acid Solution
    • 230-907-9
    • 2-(4-(2-Hydroxyethyl)piperazin-1-yl)ethanesulfonic acid
    • 2-[4-(2-hydroxyethyl)piperazin-1-yl]ethanesulfonic acid
    • 4-(2-HYDROXYETHYL)-1-PIPERAZINE ETHANESULFONIC ACID
    • 4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid
    • MFCD00006158
    • N-2-Hydroxyethylpiperazine-N'-ethanesulfonate
    • N-2-Hydroxyethylpiperazine-N'-ethanesulfonic acid
    • N-2-Hydroxyethylpiperazine-N-ethane sulfonic acid
    • NSC 166663
    • N-2-Hydroxyethylpiperazine N',2'-ethanesulfonic acid
    • 2-[4-(2-Hydroxyethyl)-1-piperazinyl]ethanesulfonic acid
    • 2-[4-(2-hydroxyethyl)piperazin-1-yl]ethane-1-sulfonic acid
    • 4-(2-Hydroxyethyl)piperazin-1-ylethanesulphonic acid
    • TVZ 7;WAS 13;NSC 166663
    • BRN 0883043
    • N-2-Hydroxyethylpiperazine-N'-2-ethanesulfonic acid
    • N-(2-Hydroxyethyl)piperazine-N'-(2-ethanesulfonic acid)
    • 2-(4-(2-Hydroxyethyl)piperazin-1-yl)ethane-1-sulfonic acid
    • 2-[4-(2-hydroxyethyl)piperazinyl]ethanesulfonic acid
    • UNII-RWW266YE9I
    • DTXSID5040382
    • HeBES
    • 4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid, HEPES, N-(2-Hydroxyethyl)piperazine-N'-(2-ethanesulfonic acid)
    • Prestwick_256
    • EINECS 230-907-9
    • HEPES, dry powder
    • HEPES buffered saline
    • 1d1p
    • 1dg9
    • HEPES, biochemical grade
    • 15(R)-hydroperoxy-EPE
    • HEPES [MI]
    • hepes, 99.5%
    • Epitope ID:190604
    • EC 230-907-9
    • SCHEMBL2417
    • 5-23-02-00379 (Beilstein Handbook Reference)
    • orb1298842
    • 2-(4-(2-Hydroxyethyl)piperazin-1-yl)ethanesulfonicacid
    • CHEMBL1232545
    • SCHEMBL29153497
    • 2-[4-(2-hydroxyethyl)-1-piperazinyl] ethanesulfonic acid
    • HEPES, >=99.5% (titration)
    • HY-D0857
    • HEPES, >=99.0%
    • s6831
    • SBB057056
    • STK033062
    • AKOS001696205
    • EBC-155071
    • FH31182
    • HEPES, >=99.0% (T)
    • HEPES-buffered saline, pH 6.5 (5X)
    • BP-31246
    • BS-17486
    • DA-64103
    • DS-015636
    • HEPES, Vetec(TM) reagent grade, 99.5%
    • 6,11-Dibromo-1,4-dioxaspiro[4.7]undecane
    • CS-0015167
    • H0396
    • NS00004939
    • ST50308651
    • ST50994550
    • EN300-100251
    • F20297
    • 4-(2-hydroxyethyl)-1-piperazine ethanesulfonate
    • 4-(2-Hydroxyethyl)-1-piperazineethane sulfonate
    • 4-(2-hydroxyethyl)-1-piperazine ethanesulphonate
    • 4-(2-Hydroxyethyl)-1-piperazineethane sulphonate
    • F047861
    • N-2-Hydroxyethylpiperazine-N-2-ethanesulfonic acid
    • Q418359
    • (4-(2-Hydroxyethyl)-1-piperazineethanesulfonic acid
    • 4-(2-hydroxylethyl)piperazine-1-ethanesulfonic acid
    • N-2 hydroxyethylpiperazine-N-2-ethane-sulfonic acid
    • N-2-Hydroxyethylpiperazino-N'-2-ethanesulfonic acid
    • 4-(2-hydroxyethyl)-1-piperazine ethanesulphonic acid
    • 4-(2-Hydroxyethyl)-1-piperazineethane sulphonic acid
    • HEPES-buffered saline, pH 7.0 (2X for transfection)
    • n-(2-hydroxyethyl)-piperazine-n'-ethanesulfonic acid
    • 4-(2-Hydroxyethyl)piperazine-1-(2-ethanesulfonic acid)
    • F0001-1858
    • HEPES, BioUltra, for molecular biology, >=99.5% (T)
    • Hepes,4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid
    • HYDROXYETHYLPIPERAZINE ETHANE SULFONIC ACID [II]
    • Z1335657480
    • 2-[4-(2-hydroxyethyl)-1-piperazinyl]ethane sulfonic acid
    • 2-[4-(2-Hydroxyethyl)-1-piperazinyl]ethanesulfonic acid #
    • 4-(2-Hydroxyethyl)-1-piperazineethanesulfonic acid (GMP)
    • hepes (4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid)
    • HEPES, anhydrous, free-flowing, Redi-Dri(TM), >=99.5%
    • 2-(4-(2-hydroxyethyl)piperazinyl)ethanesulfonic acid (HEPES)
    • N-2-HYDROXYETHYLPIPERAZINE N'-2'-ETHANESULPHONIC ACID
    • (2-hydroxyethyl)piperazine-1-ethanesulfonic acid, (HEPES) (for injection)
    • HEPES, BioPerformance Certified, >=99.5% (titration), cell culture tested
    • HEPES, BioXtra, pH 5.0-6.5 (1 M in H2O), >=99.5% (titration)
    • HEPES, Pharmaceutical Secondary Standard; Certified Reference Material
    • HEPES, BioXtra, suitable for mouse embryo cell culture, >=99.5% (titration)
    • HEPES 2-[4-(2-HYDROXYETHYL)-1-PIPERAZINYL]ETHANESULFONIC ACID MOLECULAR BIOLOGY GRADE
    • 7365-45-9

  • Applications

    4-(2-Hydroxyethyl)-1-piperazineethanesulfonic acid, CAS number: 7365-45-9, is primarily used as a biological buffering agent at near-physiological pH. In life sciences research, it is commonly employed as a buffer for cell culture experiments and for enzyme assays, and it helps stabilize proteins in storage solutions, maintaining pH stability over time. It is also used as a buffer in analytical chemistry and biomolecule separation workflows (e.g., HPLC, capillary electrophoresis) to control pH and stabilize samples. Additionally, HEPES serves as a buffering component in formulations and handling of proteins in biopharmaceutical and biotech research, subject to local regulations and formulation limits.

    gpt-5-nano

  • Solubility @25˚C

    Solvent Solubility (g/L)
    ethanol 0.45
    methanol 2.99
    isopropanol 0.13
    water 114.11
    ethyl acetate 0.18
    n-propanol 0.26
    acetone 0.16
    n-butanol 0.18
    acetonitrile 0.27
    DMF 4.55
    toluene 0.11
    isobutanol 0.15
    1,4-dioxane 0.33
    methyl acetate 0.16
    THF 0.52
    2-butanone 0.18
    n-pentanol 0.1
    sec-butanol 0.13
    n-hexane 0.02
    ethylene glycol 5.87
    NMP 21.23
    cyclohexane 0.11
    DMSO 14.4
    n-butyl acetate 0.27
    n-octanol 0.1
    chloroform 0.31
    n-propyl acetate 0.13
    acetic acid 5.75
    dichloromethane 0.37
    cyclohexanone 1.38
    propylene glycol 2.18
    isopropyl acetate 0.13
    DMAc 7.65
    2-ethoxyethanol 1.34
    isopentanol 0.14
    n-heptane 0.06
    ethyl formate 0.64
    1,2-dichloroethane 0.3
    n-hexanol 0.21
    2-methoxyethanol 4.05
    isobutyl acetate 0.08
    tetrachloromethane 0.22
    n-pentyl acetate 0.15
    transcutol 2.71
    n-heptanol 0.17
    ethylbenzene 0.08
    MIBK 0.14
    2-propoxyethanol 1.6
    tert-butanol 0.15
    MTBE 0.03
    2-butoxyethanol 0.84
    propionic acid 1.62
    o-xylene 0.27
    formic acid 63.42
    diethyl ether 0.05
    m-xylene 0.17
    p-xylene 0.13
    chlorobenzene 0.15
    dimethyl carbonate 0.41
    n-octane 0.02
    formamide 45.57
    cyclopentanone 1.44
    2-pentanone 0.14
    anisole 0.12
    cyclopentyl methyl ether 0.32
    gamma-butyrolactone 3.13
    1-methoxy-2-propanol 1.4
    pyridine 0.63
    3-pentanone 0.25
    furfural 6.2
    n-dodecane 0.01
    diethylene glycol 6.08
    diisopropyl ether 0.04
    tert-amyl alcohol 0.17
    acetylacetone 0.47
    n-hexadecane 0.01
    acetophenone 0.52
    methyl propionate 0.35
    isopentyl acetate 0.28
    trichloroethylene 0.6
    n-nonanol 0.12
    cyclohexanol 0.45
    benzyl alcohol 0.45
    2-ethylhexanol 0.2
    isooctanol 0.15
    dipropyl ether 0.15
    1,2-dichlorobenzene 0.28
    ethyl lactate 0.74
    propylene carbonate 1.68
    n-methylformamide 4.5
    2-pentanol 0.08
    n-pentane 0.02
    1-propoxy-2-propanol 0.95
    1-methoxy-2-propyl acetate 0.74
    2-(2-methoxypropoxy) propanol 1.57
    mesitylene 0.17
    ε-caprolactone 1.63
    p-cymene 0.23
    epichlorohydrin 0.88
    1,1,1-trichloroethane 0.16
    2-aminoethanol 4.17
    morpholine-4-carbaldehyde 8.33
    sulfolane 12.89
    2,2,4-trimethylpentane 0.01
    2-methyltetrahydrofuran 0.27
    n-hexyl acetate 0.16
    isooctane 0.01
    2-(2-butoxyethoxy)ethanol 1.51
    sec-butyl acetate 0.09
    tert-butyl acetate 0.17
    decalin 0.04
    glycerin 15.19
    diglyme 2.41
    acrylic acid 3.66
    isopropyl myristate 0.1
    n-butyric acid 1.07
    acetyl acetate 0.33
    di(2-ethylhexyl) phthalate 0.41
    ethyl propionate 0.17
    nitromethane 3.56
    1,2-diethoxyethane 0.17
    benzonitrile 0.43
    trioctyl phosphate 0.24
    1-bromopropane 0.09
    gamma-valerolactone 4.4
    n-decanol 0.1
    triethyl phosphate 0.63
    4-methyl-2-pentanol 0.08
    propionitrile 0.22
    vinylene carbonate 2.01
    1,1,2-trichlorotrifluoroethane 7.74
    DMS 0.39
    cumene 0.09
    2-octanol 0.08
    2-hexanone 0.1
    octyl acetate 0.13
    limonene 0.27
    1,2-dimethoxyethane 0.78
    ethyl orthosilicate 0.49
    tributyl phosphate 0.3
    diacetone alcohol 0.77
    N,N-dimethylaniline 0.25
    acrylonitrile 0.55
    aniline 0.37
    1,3-propanediol 2.36
    bromobenzene 0.12
    dibromomethane 0.24
    1,1,2,2-tetrachloroethane 0.63
    2-methyl-cyclohexyl acetate 0.53
    tetrabutyl urea 0.64
    diisobutyl methanol 0.16
    2-phenylethanol 0.59
    styrene 0.07
    dioctyl adipate 0.24
    dimethyl sulfate 2.0
    ethyl butyrate 0.34
    methyl lactate 1.9
    butyl lactate 0.64
    diethyl carbonate 0.35
    propanediol butyl ether 1.15
    triethyl orthoformate 0.44
    p-tert-butyltoluene 0.21
    methyl 4-tert-butylbenzoate 1.02
    morpholine 0.67
    tert-butylamine 0.07
    n-dodecanol 0.07
    dimethoxymethane 0.65
    ethylene carbonate 1.23
    cyrene 4.8
    2-ethoxyethyl acetate 0.27
    2-ethylhexyl acetate 0.25
    1,2,4-trichlorobenzene 0.42
    4-methylpyridine 0.38
    dibutyl ether 0.06
    2,6-dimethyl-4-heptanol 0.16
    DEF 0.9
    dimethyl isosorbide 3.42
    tetrachloroethylene 0.82
    eugenol 1.36
    triacetin 0.62
    span 80 1.2
    1,4-butanediol 0.84
    1,1-dichloroethane 0.1
    2-methyl-1-pentanol 0.1
    methyl formate 2.25
    2-methyl-1-butanol 0.16
    n-decane 0.02
    butyronitrile 0.17
    3,7-dimethyl-1-octanol 0.13
    1-chlorooctane 0.04
    1-chlorotetradecane 0.02
    n-nonane 0.02
    undecane 0.02
    tert-butylcyclohexane 0.02
    cyclooctane 0.05
    cyclopentanol 0.61
    tetrahydropyran 0.2
    tert-amyl methyl ether 0.06
    2,5,8-trioxanonane 2.59
    1-hexene 0.03
    2-isopropoxyethanol 0.47
    2,2,2-trifluoroethanol 7.57
    methyl butyrate 0.26

    Scent© AI

1 4

IFRA standard 51

Recommendation
No restriction
Maximum acceptable concentrations in the finished product (%)
Category 1
Products applied to the lips
 No restriction Category 7A
Rinse-off products applied to the hair with some hand contact
 No restriction
Category 2
Products applied to the axillae
 No restriction Category 7B
Leave-on products applied to the hair with some hand contact
 No restriction
Category 3
Products applied to the face/body using fingertips
 No restriction Category 8
Products with significant anogenital exposure
 No restriction
Category 4
Products related to fine fragrance
 No restriction Category 9
Products with body and hand exposure, primarily rinse off
 No restriction
Category 5A
Body lotion products applied to the body using the hands (palms), primarily leave on
 No restriction Category 10A
Household care products with mostly hand contact
 No restriction
Category 5B
Face moisturizer products applied to the face using the hands (palms), primarily leave on
 No restriction Category 10B
Household care products with mostly hand contact, including aerosol/spray products (with potential leave-on skin contact)
 No restriction
Category 5C
Hand cream products applied to the hands using the hands (palms), primarily leave on
 No restriction Category 11A
Products with intended skin contact but minimal transfer of fragrance to skin from inert substrate without UV exposure
 No restriction
Category 5D
Baby Creams, baby Oils and baby talc
 No restriction Category 11B
Products with intended skin contact but minimal transfer of fragrance to skin from inert substrate with potential UV exposure
 No restriction
Category 6
Products with oral and lip exposure
 No restriction Category 12
Products not intended for direct skin contact, minimal or insignificant transfer to skin
 No restriction